Author(s): Jie, JY (Jie, Junyao); Hu, SM (Hu, Shiming); Liu, WW (Liu, Wenwen); Wei, QQ (Wei, Qingquan); Huang, YZ (Huang, Yizheng); Yuan, XX (Yuan, Xinxin); Ren, LF (Ren, Lufeng); Tan, MQ (Tan, Manqing); Yu, YD (Yu, Yude)

Source: SENSORS Volume: 20 Issue: 9 DOI: 10.3390/s20092627 Published: MAY 2020

Abstract: Polymerase chain reaction (PCR) is a technique for nucleic acid amplification, which has been widely used in molecular biology. Owing to the limitations such as large size, high power consumption, and complicated operation, PCR is only used in hospitals or research institutions. To meet the requirements of portable applications, we developed a fast, battery-powered, portable device for PCR amplification and end-point detection. The device consisted of a PCR thermal control system, PCR reaction chip, and fluorescence detection system. The PCR thermal control system was formed by a thermal control chip and external drive circuits. Thin-film heaters and resistance temperature detectors (RTDs) were fabricated on the thermal control chip and were regulated with external drive circuits. The average heating rate was 32 degrees C/s and the average cooling rate was 7.5 degrees C/s. The disposable reaction chips were fabricated using a silicon substrate, silicone rubber, and quartz plate. The fluorescence detection system consisted a complementary metal-oxide-semiconductor (CMOS) camera, an LED, and mirror units. The device was driven by a 24 V Li-ion battery. We amplified HPV16E6 genomic DNA using our device and achieved satisfactory results.

Accession Number: WOS:000537106200191

PubMed ID: 32380637

Author Identifiers:

Author        Web of Science ResearcherID        ORCID Number

Ren, Lufeng                  0000-0002-4098-3668

eISSN: 1424-8220

Full Text: https://www.mdpi.com/1424-8220/20/9/2627